Supplies Needed

• 3 lb All-In-One grow bag (with injection port)

• Sterile liquid culture syringe (10mL)

• Alcohol prep wipes (2 per bag)

• Gloves (recommended)

• Face mask (recommended)

• Lighter (for sterilizing needle, if needle is sterile this is optional)

• Clean environment (preferably still air box or sanitized area)

   

Colonization Timeline

• Grain colonization: ~7–14 days

• Substrate colonization (after shaking or mixing): ~7–14 days

• Wait until the bag is fully white (colonized) before fruiting.

   

Ideal Conditions

• For colonization 70-75f

• For fruiting 68-72f

   

Step-by-Step Instructions

1. Prepare Your Workspace

• Clean your work surface with 70% isopropyl alcohol.

• Wash your hands thoroughly.

• (Optional but recommended) Wear gloves and a face mask.

• Ensure the bag is upright and the injection port is clearly visible.

   

2. Sanitize Everything

• Wipe the injection port with an alcohol wipe.

• Wipe the LC syringe body (not the needle) with second alcohol wipe.

• Make sure everything stays as clean as possible from this point forward.

   

3. Sterilize the Needle (the needle provided with Psychedelic Goat kits are sterile and this is optional)

• Remove the syringe cap.

• Flame the needle tip with a lighter or alcohol lamp until it glows red.

• Let it cool for 5–10 seconds. Do not set it down or blow on it.

   

4. Inject the Liquid Culture

• Insert the needle into the center of the injection port (the white rubber circle).

• Inject 5-10 mL of liquid culture.

• Withdraw the needle carefully and recap it if you plan to save for further use.

   

5. Label and Store

• Write the date and strain on the bag.

• Store the inoculated bag in a clean, dark place at 70–75f

• Avoid moving or squeezing the bag for the first 7–10 days.

   

6. Massage the Bag (Just the grains)

• Once a quarter to half dollar size spot of mycelium appreas, massage the grains.

• Avoid breaking the separation between grain and substrate layers if they’re clearly divided.

   

7. Mix the Bag

• When all the grain in completely colonized, mix the grain with the substrate.

   

8.Fruit the Bag

• Make sure the bag is completely colonized and pins start to appear

• Cut bag off about one inch up from the bottom.

• Mist walls of bins as necessary.